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#DETERMINATION OF PARTITION COEFFICIENT OF BENZOICACID ACID BETWEEN OIL AND WATER

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Aim :   To determine the partition coefficient of benzoic acid between oil and water. Apparatus:     Separating funnel, burette, pipette, conical flask, Beaker. Chemicals used:     0.1N NaOH solution, potassium hydrogen phthalate, benzoic acid, peanut (or) arachis oil, water, phenolphthalein indicator. Theory:          Nernst distribution law states that when added substance is insufficient to saturate immiscible liquids the solute distributes between liquids. In such a way that at equilibrium the ratio of concentration of the solute on the two liquids is constant at constant temperature partition coefficient may be defined as the ratio of solute in organic phase to that of aqueous phase.IfC 0 and are C W equilibrium concentration of substance in organic phase and aqueous phase respectively then the expression of                                                K d = C 0 /C W Where the equilibrium constant k d is called partition coefficient   This equation a
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#ASSAY OF FUROSEMIDE

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Aim: To report the amount of Furosemide present in the given tablet/powder/solution . Materials : Burettes, pipettes, beakers, glass rod, funnel etc Chemicals : Procedure :1 Sodium hydroxide, PHP, chloroform, furosemide drug, DMF, Phenolphthalein indicator Procedure: 2 Potasium Bromate,Potasium bromide,HCl,Methyl orange Principle:              It is assayed by alkalimetry. The sample is dissolved in Dimethyl formamide and titrated against standardized solution of sodium hydroxide using bromothymol blue solution as an indicator.                   Procedure: Standardization of 0.1M Sodium Hydroxide Weigh accurately 0.2gm of Potassium hydrogen phthalate (C 8 H 5 KO 4 ) in to a conical flask. Dissolve it in 10ml of distilled water and add 2 drops of phenolphthalein indicator. Titrate this solution with prepared 0.1M NaOH solution until a permanent pale pink colour was produced.               Each ml of 0.1M NaOH ≡ 0.02042 gm of C 8 H 5 KO 4 .  
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#ASSAY OF PHENOBARBITONE

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Aim: To determine the percentage purity of phenobarbitone present in the given powder. Apparatus: Burette, Pipette, Burette stand, conical flask. Chemicals:   Silver nitrate, phenobarbitone, sodium carbonate, potassium chromate indicator. Principle:           Assay of phenobarbitone comes under precipitation titration. Barbiturates when interact with silver nitrate in the presence of sodium carbonate initially it forms one substituted sodium salt of phenobarbitone, which reacts with silver nitrate (AgNO 3 ) with the formation of soluble one-substituted Ag-salt. Then soluble Na-Ag-salt is formed. In equivalence point excess of titrant AgNO 3 destroys Na-Ag-salt and the insoluble two-substituted Ag-salt of phenobarbitone is formed, that indicates the end point. Procedure: Standardisation of Silver nitrate: Weigh accurately 0.5g (5ml) of NaCl and add 10ml of water in to conical flask. Add 0.5ml of indicator and titrate against AgNO 3 solution until brick red colo
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